Correlative Raman imaging: New insights for bio-mineral and nano materials

New insights-bio-mineral-nano materials

Correlative Raman imaging: New insights for bio-mineral and nano materials

AFM topography (top) and Raman (bottom) images of the cuticle of a Crustacea. The Raman image reveals the distribution of amorphous Calcium carbonate phases (red & blue) along with additional organic compounds (green).
Correlative Raman imaging: New insights for bio-mineral and nano materials

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In nature, biomineralization is a widespread and evolutionarily ancient phenomenon. It’s the molecular basis for the formation of pearls, bones, exoskeletons of crustaceans, teeth, molluscan shells, kidney stones and many other biological structures. To build structural features organisms from bacteria to humans use many varieties of minerals such as hydroxyapatite, calcium carbonates and phosphate, silica and magnetite.

Raman Imaging in combination with Atomic Force Microscopy (AFM) or Scanning Electron Microscopy (SEM) can provide new insights into the fundamental processes by which organisms produce biocomposites containing crystallized minerals. After an understanding of the mechanisms of biomineralization is obtained, it should be possible to  exploit them in technical applications.

In this webinar we will first introduce the principles of state-of-the-art confocal Raman imaging as a tool for analyzing the chemical and molecular characteristics of a sample.

Then we will show how this technique can be used in combination with AFM and SEM to correlate  chemical information with structural features. Altogether it will demonstrate the advantages  of microscopy systems that integrate Raman-AFM and Raman-SEM (RISE), respectively, in standalone hybrid instruments.

  • Hear from a leading manufacturer of confocal Raman microscopes how to perform Raman imaging and interprete Raman data.
  • Understand the remarkable potential of correlative Raman-AFM and Raman-SEM approaches using “all-in-one” instrumental setups for investigating complex organic materials.
  • Learn about the application of Raman imaging for the characterization of mineralized structures of organisms.


Dr. Ute Schmidt, Applications Manager at WITec GmbH
Joe D’angelo, (Moderator), Materials Science Publisher.

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