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Sphere Fluidics Picodroplet Single Cell Encapsulation System

Cyto-Mine® – Antibody discovery and cell line development workflows streamlined like never before

  • Targeted Discovery – .Rapidly identify and isolate your rare cells of interest.
  • Monoclonality Assurance – Extensive proof of monoclonality for cell line development.
  • Significantly Reduce Timelines and Costs – Tens of millions of cells in pools or hundreds of thousands individually are screened in a single day.
  • Flexibility – Flexible assay design allows quantitative measurement of antibody secretion.
  • Sterility – The entire process is automated, sterile and Animal Origin Free. The Benchtop system is compatible for use in Class II biosafety cabinets.

 

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Introducing the Sphere Fluidics Picodroplet Single Cell Encapsulation System

Our semi-automated system encapsulates single cells or biomolecules into picodroplets, ready for downstream screening and analysis. It does this at a rapid rate of up to 70,000 picodroplets per second, and the flow rate can be highly controlled. The instrument enables rapid and very sensitive detection of secreted or cell-associated proteins produced by an individual cell, contained in each picodroplet.

The encapsulation system doesn’t affect cell viability, while the wide range of picodroplet sizes and volumes gives it flexibility for use with various cell types – large or small. These picodroplets can be stabilised using novel surfactants and cells can be grown in them, and even incubated or stored for many days.

 

Sphere Fluidics Picodroplet Single Cell Encapsulation System



Sphere Fluidics Picodroplet Single Cell Encapsulation System

Key Features

A flexible and easy-to-use instrument to enhance your single cell research capabilities

Our research instruments are designed to assist you in finding highly valuable and rare biological variants among vast cell populations. By increasing speed and reducing cost, our picodroplet technology can help you save resources whilst boosting your chances of success.

Unlike our industrial instruments, our research instruments are semi-automated rather than fully automated. While this means they require slightly more user input to configure, it also means that they are more flexible and can be easily adapted to fit the needs of your unique research project.

We currently have two research instruments that enable you to generate, isolate, and dispense picodroplets for a range of applications. These are the Picodroplet Single Cell Encapsulation System and the Picodroplet Single Cell Assay and Isolation System. Both are compatible with our range of microfluidic chemicals and biochips .

Key Features

  • Semi-automated, picodroplet generator.
  • Encapsulation of single cells or biomolecules in picodroplets.
  • High-speed generation of picodroplets (up to 70,000/sec).
  • Parallel incorporation of probes and cells in picodroplets enables sensitive detection of secreted proteins (e.g. antibodies, growth factors, cytokines, enzymes, etc.),
  • User defined microfluidic flow rates.
  • Optical imaging of picodroplet generation for QA/QC.
  • Wide range of picodroplet sizes and volumes.

 

 



Key Benefits

Some common research areas where our instruments have been used previously include (but are not limited to):

Primary B Cell Analysis

Recent developments antibody drug discovery, through single cell gene sequencing and recombinant immunoglobulin expression, have enabled direct screening of native B cells (B lymphocytes), giving the potential for much more of the mammalian immune system repertoire to be deep-mined. Our Research Instruments allow you to rapidly screen vast numbers of native B cells to find rare cells of interest.

Hybridoma Screening

Hybridoma generation and screening is a powerful tool in antibody drug discovery. Current techniques used in the field are restricted by the number of hybridomas they can screen each day, slowing down pipelines. Our Research Instruments enable the analysis of hybridomas in picodroplets, allowing for a more flexible, controlled and rapid approach.

Drug resistance studies

Drug resistance is a growing concern worldwide. The picodroplet approach enables phenotypic screening of extraordinarily large numbers (billions per day) of bacteria to identify those which have developed resistance to the drug of interest. In collaboration with Merck, we demonstrated that microfluidic-based picodroplet platforms enable effective and high-throughput assessment and isolation of antibiotic-resistant bacteria in a label-free manner.

Enzyme evolution

There is a growing focus in evolutionary biochemistry on leveraging big data and high-throughput experimental tools to uncover the expansive functional diversity of enzymes and the evolutionary processes driving it. Picodroplet technology provides a novel, flexible and rapid method for analysing enzyme evolution in a high-throughput and controlled manner.

Cell therapy

There is a growing interest and investment in T-cell immunotherapies, as they provide hope for future treatments of life-threatening diseases such as blood cancers. T-cell therapies capitalise on the body’s immune system to destroy tumour cells by collecting a patient’s own T-cells and modifying them to target cancer cells, by transferring DNA into the cells using viral vectors. The vector transfer efficiency and its consistency are critical to the final (cell) product, quality and cost. We have engineered new microfluidic-based technologies to improve the efficiency of transferring DNA into cells.

Synthetic biology

The Research Instruments are ideally suited for the rapid, miniaturised and high-throughput profiling of biosynthetic libraries. One example where we carried out this type of work was in collaboration with Imperial College London, where we combined microfluidic-based picodroplet technology with Mass Spectroscopy for the rapid screening, identification and retrieval of the best “hit” cells among synthetic metabolic pathway libraries.

 


Sphere Fluidics Picodroplet Single Cell Encapsulation System


Trust the Experts at Spectra Research Corporation

  • Semi-automated, picodroplet generator.
  • Encapsulation of single cells or biomolecules in picodroplets.
  • High-speed generation of picodroplets (up to 70,000/sec).
  • Parallel incorporation of probes and cells in picodroplets enables sensitive detection of secreted proteins (e.g. antibodies, growth factors, cytokines, enzymes, etc.),
  • User defined microfluidic flow rates.
  • Optical imaging of picodroplet generation for QA/QC.
  • Wide range of picodroplet sizes and volumes.

 

Specifications

SYSTEM SPECIFICATIONS
Sample input format Syringe pumps
Sample input volume ~ 50 µl – 1 mL
Workflow Picodroplet production and retrieval
Detection system n/a
Picodroplet volume 30 pL – 1 nL
Throughput Generation rate: 100 – 5,000 per second
SYSTEM SPECIFICATIONS
Biochip compatibility Pico-Gen™ picodroplet biochips
Weight (approx.) 50 kg (110 lbs)
Dimensions (approx.) 130 cm x 60 cm x 60 cm (width x height x depth)
Voltage [frequency] 100 V (min) to 240 V (max) [@ 50 / 60 Hz]
Consumption 300 W (max)
OPTICS
Optical source Halogen lamp (white light)
Detection Filters n/a
Detection Wavelengths n/a
PMT Spectral Sensitivity n/a
Camera High-speed CMOS (1,696 pixels x 1,710 pixels), (500 fps at full resolution, up to 200,000 fps at reduced resolution)
Computer Dell Optiplex 3020 (4 GB RAM; 500 GB hard drive) or equivalent
PC Operating System Microsoft Windows 7 Professional SP1 or newer version
Monitor Colour LCD (21”)
External connections 2 USB, 1 Ethernet
System Control Software neMESYS Syringe Pump software, camera software
Data Format n/a
WORK ENVIRONMENT
Clearance 30 cm
Operating Temperature 21°C ± 5°C
Site preparation n/a

 

Datasheet


Contact Spectra Research

Address

3585 Laird Rd., Unit 15 & 16,
L5L 5Z8 Mississauga
Ontario
Canada

Phone :  905-890-0555

Toll Free : 1-866-753-4433
Fax :  905-890-1959

Email: info@spectraresearch.com

About Spectra Research

Spectra Research Corporation (SRC) offers a range of innovative high-quality scientific products and laboratory services to industrial and scientific markets throughout Canada.

Established in 1993, SRC is a subsidiary of Allan Crawford Associates (ACA), one of Canada’s largest distributors of electronic components, test equipment and integrated networking solutions.

Spectra Research Location

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