NTNU’s NanoLab facility uses NanoSight NTA system to characterize nanoparticles.

NanoSight, leading manufacturers of unique nanoparticle characterization technology, reports on the characterization of nanoparticles at the NanoLab of the Norwegian University of Science & Technology, NTNU.

NTNU NanoLab is a cross faculty, strategic initiative with the objective to coordinate and promote nanoscience and nanotechnology at NTNU. The laboratories are well-equipped with state of the art instrumentation designed to be used by as many researchers from as many disciplines as possible.

For example, Katarzyna Psonka- Antonczyk is a post-doctoral fellow in the Biophysics and Medical Technology group within the Department of Physics. Her interests include the characterization of nanovesicles (exosomes) with sizes ranging from 30 to a few hundred nanometers secreted by cancer cells to the extracellular matrix. Exosomes are intercellular shuttle vehicles of various materials and contain information that can reprogram targeted cells. Exosomes contain membrane proteins, cytosolic proteins and small RNAs (miRNA). These vesicles are transported by bodily fluids (blood) and can likely fuse back with plasma membranes, introducing new proteins and RNA in new cells distant from the cell of origin. She is applying single-molecule techniques like atomic force microscopy and total internal reflection fluorescence microscopy to visualize individual exosomes and to characterize their membrane repertoire.

Knowing the concentration of secreted exosomes can facilitate estimation of the secretory abilities of cells and can help in further sample preparation. When coupled with fluorescent light, the NanoSight Nanoparticle Tracking Analysis system enables the analysis of exosome samples to provide information of various distinct subpopulations of vesicles by labeling with specific antibodies tagged with a fluorescent reporter. In the opinion of Dr Psonka-Antonczyk, the NanoSight LM10 has proven to be a very suitable instrument to access the concentration and size profile of exosomes.

Prior to using NanoSight, Dr Psonka-Antonczyk tried to employ dynamic light scattering but the results were rather irreproducible and not very reliable. In contrast, NanoSight exceeded her expectations. She said “NanoSight’s system is simple and easy to operate in providing information on the exosomes concentration and size profile in a very short time. I can also use it as a test measurement providing the first glance on the exosomes before running more elaborate and time-consuming experiments.”
The range of applications for NTA at NanoLab is diverse. For example, metallic and magnetic nanoparticles (Chemical Engineering) used for biomedical applications including targeted drug delivery and MRI contrast enhancement are studied by Dr Gurvinder Singh. He likes the NTA approach because “it provides the determination of particle concentration, better resolution of particle size and size distribution with real time visualization. This instrument is more impressive than DLS.”

Calorimetry As A Key Technique For Gas Adsorption Investigation

The investigation of gas adsorption on catalysts and solid sorbents depend upon a good interaction between the reactive gas and the powder. The SENSYS evo DSC is equipped with a crossing furnace, which allows the use of a quartz tube reactor. This makes it possible to operate in true plug-flow fixed bed reactor mode, widely used in heterogeneous catalysis, and thus precisely simulate industrial processes.

Additionally the exhaust gases can be flowed to an on-line gas analyzer such as a mass spectrometer, TCD or FTIR analyzer.

More complex thermal and gas flow profiles can provide adsorption / desorption / temperature programmed desorption data from a single experiment.

Example of application

  • Determination of the heat of adsorption / desorption of NH3 on different solid media. Simultaneous determination of the amounts of NH3 adsorbed / desorbed with coupled quantification techniques.
  • Catalytic oxidation of propane

Evaluation of the performance of catalysts for the selective catalytic reduction (SCR) of NO with NH3 and O2

See more in the following Aplication Note AN674 -Gas adsorption on catalysts and adsorbents using a quartz tube reactor on a Calvet type DSC

calorimetry

Introducing the New Environmental Scanner for the Cypher™ AFM – the Highest Resolution Fast Scanning AFM

Imagine an AFM that routinely images the DNA double helix (top image) and atomic point defects (bottom) – in any environment.

We invite you to Booth 510 at the MRS Fall Meeting for an exclusive preview of the new Environmental Scanner for Cypher.

The Cypher Environmental Scanner is a modular and customizable platform with sample temperature control in a wide spectrum of environments. The Environmental Scanner is designed around a sealed cell which can sustain moderate positive pressures and uses chemically inert materials, such as fused silica and FFKM, to provide a controlled environment while maintaining accurate and distortion free imaging.

Cypher offers the widest range of scanning environments:

  • Buffers, solvents, inert gases, strong acids/bases
  • Liquid/gas perfusion
  • Continuous liquid flow
  • Temperature control from 0°C to 250°C
  • Optional electrical feedthroughs

If your experiments require a glove box, Cypher can also be operated in this configuration.

MFP-3D™ AFM – High Resolution,
Closed-loop Precision, and Flexibility

We’ll also be featuring the MFP-3D, the most versatile AFM platform with numerous environmental accessories and advanced scanning modes. The MFP-3D can be configured as:

Both the MFP-3D and Cypher AFMs scan with all standard AFM imaging modes (tapping, contact, MFM, phase, etc.), and numerous exclusive imaging modes: