The Analytical Power of Correlative Raman Imaging: New Developments, Tools and Applications

Analytical Power

Topic  

The Analytical Power of Correlative Raman Imaging: New Developments, Tools and Applications
Description

Confocal Raman imaging is a powerful, versatile and increasingly common microscopy technique, capable of quickly identifying the molecules in a sample and visualizing their physical distribution three-dimensionally. This nondestructive and label-free chemical imaging method has enormous potential for researchers in many fields of application.

This webinar will introduce the fundamental principles of Raman microscopy and it will detail the associated hardware and software. Our speakers will describe several of its variations providing relevant application examples. Correlative microscopy will then be introduced, and details of Raman-AFM and Raman-SEM (RISE) microscopy solutions will be presented.

Key Learning Objectives
During this webinar, the speakers will present:

  • What Raman microscopy is, along with an introduction to its operational principles and hardware considerations.
  • Possibilities and new tools for Raman investigations including several application examples from various fields.
  • How correlative microscopy can provide a more complete understanding of a sample than techniques in isolation.
Time

Sep 10, 2020 11:00 AM in Eastern Time (US and Canada)

Speakers

photo of Dr. Miriam Böhmle
Dr. Miriam Böhmle
Senior Applications Scientist @WITec GmbH
Dr, Miriam Böhmler is a Senior Applications Scientist at WITec GmbH in Ulm, Germany. She possesses wide-ranging expertise in Raman imaging and spectroscopy, near-field and super-resolution optical technologies such as tip-enhanced Raman spectroscopy, and nano-FTIR spectroscopy.
photo of Dr. Ute Schmidt
Dr. Ute Schmidt
Applications Manager @WITec
Dr. Ute Schmidt is an Applications Manager at WITec. She studied physics at the Babes Bolyai University in Cluj-Napoca, Romania, and obtained her PhD from the University of Karlsruhe, Germany. Through her work with in-situ scanning tunneling microscopy, Atomic Force Microscopy and SEM, Schmidt is an expert for correlative imaging techniques.

 

Correlative Raman Imaging

The Next Tool in Surface Metrology Introducing the S wide

SRC is pleased to introduce Sensofar Metrology’s new metrology tool for wide areas—the S wide. The S wide is a dedicated system designed to rapidly measure large sample areas up to 300 x 300 mm. It provides all the benefits of a digital microscope integrated into a high-resolution measuring instrument. On top of that, with single button acquisition, it is extremely easy to use.

S wide

Large-area 3D optical system

 

The S wide is a large-area 3D optical system providing solutions in the following fields:

  • Advanced manufacturing
  • Archaeology and paleontology
  • Consumer electronics
  • Medical devices
  • Molding
  • Optics
  • Watches

S wide features

  • Sub-micron height repeatability over entire extended area
  • One shot height measurement up to 40 mm without Z scanning
  • Bi-telecentric lenses with very low field distortion providing accurate metrology
  • Form deviation from 3D CAD models, providing the geometric difference and tolerance measurement

 

S wide

ISO standards

Traceability

Every S wide is manufactured to deliver accurate and traceable measurements. Systems are calibrated using traceable standards according to ISO 25178 and VDI 2634-2. 

Request a quote

Click here to request a quote on a Sensofar Group product.

About Sensofar Metrology

Sensofar Metrology is a member of the Sensofar Group, headquartered near Barcelona, a technology and innovation hub. Sensofar Metrology’s mission is to develop, manufacture and market high-end 3D surface metrology instruments. They also provide consultancy within the field of metrology, and pursue a philosophy of guaranteeing advanced techniques, high quality and customer service.

 

FREE WEBINAR FROM ASYLUM RESEARCH on Application of Atomic Force Microscopy in Virology Research

WEBINAR DATE

Wednesday August 12, 2020
12:00 PM – 1:00 PM EDT

Atomic Force Microscopy has found broad use in the biological sciences. It is an indispensable tool for both high resolution imaging of individual molecules, cells, tissues, viruses and biomaterials. Since AFM is also relatively non-destructive and requires minimal sample preparation (i.e. no coating/fixing/labeling), it is ideally suited to visualize
dynamic biomolecular processes at the nanoscale.
We will present relevant data to illustrate the potential of Asylum Research’s AFMs in virus research.

 

Sophia Hohlbauch, Senior Biological Applications Scientist @Asylum Research
Sophia Hohlbauch has over 20 years of AFM experience specializing in the life sciences. She is currently a Senior Biological Applications Scientist at Asylum Research and previously held a similar position at Digital Instruments/Veeco. She has presented on AFM topics at numerous biology and AFM meetings worldwide. Her background is biology, receiving Bachelor’s and Master’s degrees in Marine Biology from the University of California, Santa Barbara.

Sensofar Four4Free webinars in June!

As we did during the month of April, we would like to share a virtual coffee with you again this coming June.

Join our Four4Free weekly webinars to hear best-in-class metrology tips from our most experienced experts. This month we are focusing on a more technical field to get the most out of optical profilers in different applications.

The live events will take place every Thursday in June, one full hour of exciting keynotes and will allow time for your questions too.

We want to bring our knowledge to meet your new ideas!


OUR FIRST APPOINTMENT  
Learn more about the unlimited world of surface metrology

Sensofar Four4Free webinars

REGISTER NOW

NEXT WEEK!

WEBINAR | 4 JUN

Optical Metrology Masterclass II:
Be an Expert on Thickness & Interferometry

A second chance to learn everything we know

Our most renowned expert, Dr. Roger Artigas CTO & President of Sensofar Metrology, will dive deep into the features that identify Thickness & Interferometry optical measurement technologies to solve any challenge or question you may have regarding the use of them.

Test your Interferometry skills!

WEBINAR | 11 JUN

Tribology,
measuring surfaces effectively

READ MORE   

 

June Four4Free

WEBINAR 18 JUN

WEBINAR | 18 JUN

Why an Optical Profileris crucial for Paleontology & Archaeology

READ MORE
 

WEBINAR | 25 JUN

ISO 25178:
Surface Texture Characterization

READ MORE

WEBINAR 25

 

Rokit Healthcare Dr. INVIVO 4D6 3D Printing system

Dr. INVIVO 4D6 3D

Topic

SRC: Rokit Healthcare Dr. INVIVO 4D6 3D Printing system

Description

We will share the vision behind the Dr. INVIVO 4D6 development, from creating novel personalized medicines based on bioprinting to revolutionizing the healthcare supply chain through in-hospital manufacturing.

The seminar reinforces the significance of bioprinting advancements in the age of Industry 4.0 – as a movement aligned with global megatrends in healthcare toward personalized medicine, computer-aided production of biological processes and autologous regenerative therapy.

ROKIT Healthcare believes bioprinting lends a promising, innovative solution from the convergence of biology, engineering, physics, artificial intelligence, tissue engineering, medicine and excellent management skills.

Time

Please choose only one webinar to attend.

Jun 9, 2020 04:00 PM Jun 11, 2020 04:00 PM Time shows in 

 

Reichert SPR – A Vital Tool in Developing Therapeutics and Designing Vaccines

1 –Technical note from Reichert Technologies, 3362 Walden Ave., Suite 100, Depew, NY 14043

INTRODUCTION

Surface Plasmon Resonance (SPR) is an invaluable technique that generates information-rich data for a variety of biomolecular interactions. Researchers utilize SPR to understand biological pathways and to develop and characterize a range of potential therapeutics to treat disease and other illnesses.   In the context of the current coronavirus global pandemic, SPR can be employed to develop potential therapeutics to treat COVID-19 disease and help engineer vaccines to prevent virus infections.  It can reveal vital structural and function information between the pathogen and host immune system that is critical in therapeutic development.  SPR can be used to explore a variety of potential therapeutics that include low molecular weight compounds or drugs, peptides, proteins, and antibodies.

REICHERT SPR IN MALARIA VACCINE DEVLOPMENT

The importance of using SPR in guiding vaccine development is highlighted in two recent articles where researchers used a Reichert SPR system to investigate Malaria treatment.1,2  An integral step in malaria vaccine development has been to understand how infection by parasites occurs. Of the known human malaria parasites, the group whose research is highlighted here has targeted two – Plasmodium falciparum (P. falciparum)1, which is the most deadly parasite, and Plasmodium vivax (P. vivax)2 which is the most common parasite species that infects humans. A key question that needed to be answered early on is – How do parasites gain entry into red blood cells? This group discovered that P. vivax enters young red blood cells by making proteins that recognize and bind to receptors on the young red blood cell surface. This family of proteins was structurally similar to those used by P. falciparum to infect red blood cells. The next step in their research effort was to determine the three-dimensional structure of the proteins. This determination showed that the proteins are folded in the same way with the main difference being in the electrical charge on the surface of the molecules.

Researchers found that P. falciparum reticulocyte binding protein-like homologue 4 (PfRh4) binds to complement receptor 1 (CR1 or CD35) to mediate entry of malaria parasites into human red blood cells1. CR1 (complement receptor 1) is composed of 28–30 structural modules (which are called complement control protein (CCP) domains) in the extracellular domain.  The CR1 protein fragments in the study outlined here consisted of only two CCP domains and had molecular weights of around 14,000 Daltons. Initial mapping studies identified the first two to three N-terminal modules of CR1 (CCPs 1-2 or CCPs 1–3) as specific inhibitors of the PfRh4-CR1 invasion pathway1. Seven different mutant proteins were tested for their inhibitory potential via SPR.  SPR was able to rank them in terms of affinity with three having the highest affinity for the intended target. The SPR results matched with the expected biological activity determined independently with other techniques1. Binding affinities for a series of protein fragments to PfRh4 were determined.  An example of responses from one of the higher affinity fragments is shown in Figure 1 (CR1 CCP 1-2).  Data is fit to a 1:1 binding model with mass transport model:

Reichert SPR.PNG

 

Figure 1: The following concentrations of the CR1 CCP1-2 inhibitor were injected over immobilized PfRh4:  5, 10, 20, 40 and 80 nM. Tracedrawer (Ridgeview Instruments AG) was used to fit the data.  The calculated ka is 2.10 e6 M-1 s-1, the kd is 1.26 e-2 s-1, and the equilibrium dissociation constant KD is 6.01 nM.

This information is an important starting point that will lead to further screening of potentially more powerful inhibitors and can aid in the rational development of a novel malaria vaccine. 

 

 

 

REICHERT SPR IN HIV/AIDS THERAPEUTIC DESIGN

Treatment with antiretroviral drugs is the accepted approach to HIV/AIDS therapy.  Drugs are usually taken in combinations that include nucleoside reverse transcriptase inhibitors, which block how the HIV genetic material is used to create DNA from RNA, protease inhibitors, where the raw protein material for newly produced HIV viral particles is cut into specific pieces, and integrase inhibitors, which prevent the proviral HIV DNA from integrating into the host cell genome.  These drugs do not provide a cure but significantly help slow the disease’s progression although with significant side-effects.  Researchers in this current study (3) ultimately would like to develop a new class of antiretrovirals that can interfere specifically with the functions of HIV1/Nef.  Since Nef is critical for HIV-1 replication in vivo and the immune escape of HIV-infected cells which leads to AIDS progression, development of potent HIV-1/Nef inhibitors hold promise in the suppression of HIV replication and the restoration of immune recognition of HIV-positive cells so that the patient’s own CTL response can be used to combat the disease.3  In this current work, Hydrogen Exchange Mass Spectrometry (HX MS) and SPR have been the primary techniques used to better understand the mechanism of action on Nef conformational transitions in solution by focusing on binding of Src-family kinases.  Hence, this study supports the broader possibility that compounds interfering with conformational transitions in HIV-1/Nef structure have potential as a new class of antiretroviral agents.3 This is important as HIV can become resistant to existing treatments over time and new treatments will continue to be needed.

Researchers were interested in knowing whether binding properties changed depending on whether the Nef protein was full-length or truncated so that only the folded core lacking the N-terminal arm responsible for membrane anchoring (amino acids 1-60) was present.  They also investigated a mutant defective for homodimer formation, in which Nef Asp123 was changed to Asparagine (Nef-D123N). Substitution of this highly conserved Aspartate prevented dimerization in cell-based fluorescence complementation assays and blocked important Nef functions related to infectivity and replication, as well as CD4 and MHC-I downregulation.3

After immobilization of SH3 or SH3-SH2 domains of Src-family kinases ligand, Nef protein was flowed over the chip either as full- length analyte, wild type core or D123N core.  Examples of responses seen for the wild type Nef core are shown below in Figures 2 and 3.  When SH3 alone was coupled, the wild type Nef core exhibited single-site binding with a KD value of of 128 nM.  Binding was similar for the other Nef proteins – the affinity was a little lower for the full-length Nef (180 nM) and a little higher for the D123N core (119 nM).  When SH3-SH2 was employed as the ligand, 2-site binding (conformational change) was seen with an affinity of 475 nM for the Nef core and similar affinities for the other Nef proteins (429 nM and 475 nM for the full-length Nef and D123N core, respectively).  Hence, it was found that the mutation had little impact on the kinetics or affinity of SH3 domain interaction, suggesting that the D123N mutation does not influence the conformation of Nef to inhibit binding.  This finding was consistent with HX MS results.3

SH3 protein bindingFigure 2: SH3 protein binding to Nef wild type core fit to a 1:1 binding model. Kinetic Rate constants determined in Tracedrawer are as follows: ka = 2.24 x 105 M-1s-1 and kd = 2.76 x 10-2 s-1 with an equilibrium dissociation constant (KD) of 128 nM.

Figure 3: SH3-SH2 proteins binding to Nef wild type core fit to a 2-state conformational change model. Kinetic rate constants determined in Tracedrawer are as follows: ka1 = 6.29×104 M-1s-1, kd1 =5.65×10-2 s-1 and ka2 =1.06×102 M-1s-1, kd2 = 4.72×10-3 s-1 with an equilibrium dissociation constant of KD of 475 nM.

REICHERT SPR IN MONOCLONAL THERAPEUTIC ANTIBODY DEVELOPMENT

Transmembrane 4 superfamily member 5 protein (TM4SF5) is an important possible target for hepatocellular carcinoma and colon cancer.  In this work, researchers utilized a cyclic peptide that mimics a structural motif of TM4SF5 as an antigen and then developed a monoclonal antibody that recognizes the TM4SF5 protein.4  The researcher employed Reichert SPR to characterize the antibody and showed that it has a very slow off rate making it an attractive antibody for potential therapeutic outcome.  In this study, it was shown that both the cyclic vaccine and the humanized anti-TM4SF5 antibody suppressed the formation and growth of lung metastases that were established through intravenous injection of colon cancer cells in a mouse metastases model.4 

CONCLUSION

SPR can play a pivotal role in therapeutics development and vaccine design.  Reichert’s SPR systems have been implemented in a variety of research application (see https://www.reichertspr.com/publications/) and have the sensitivity and performance characteristics to meet challenging experimental needs.   Reichert offers two main SPR platforms, the 2SPR and 4SPR systems that are affordable, flexible and have outstanding performance.  Please contact us to learn more about how Reichert SPR can help you with your therapeutic and vaccine research during this critical time. 

REFERENCES

  • Nicholas T. Y. Lim, Markus J. Harder, Alexander T. Kennedy, Clara S. Lin, Christopher Weir, Alan F. Cowman, Melissa J. Call, Christoph Q. Schmidt and Wai-Hong Tham, Characterization of Inhibitors and Monoclonal Antibodies That Modulate the Interaction between Plasmodium falciparum Adhesin PfRh4 with Its Erythrocyte Receptor Complement Receptor 1,” J. Biol. Chemistry (2015) Vol. 290, pp. 25307-25321, doi:10.1074/jbc.M115.657171. 
  • Jakub Gruszczyk, Usheer Kanjee, Li-Jin Chan, Sébastien Menant, Benoit Malleret, Nicholas T. Y. Lim, Christoph Q. Schmidt, Yee-Foong Mok, Kai-Min Lin, Richard D. Pearson,,10 Gabriel Rangel, Brian J. Smith, Melissa J. Call, Michael P. Weekes, Michael D. W. Griffin, James M. Murphy, Jonathan Abraham, Kanlaya Sriprawat, Maria J. Menezes, Marcelo U. Ferreira, Bruce Russell, Laurent Renia, Manoj T. Duraisingh, Wai-Hong Tham1, “Transferrin receptor 1 is a reticulocyte-specific receptor for Plasmodium vivax,” Science (2018), Volume 359, pp. 48-55. 
  • Jamie A. Moroco, John Jeff Alvarado, Ryan P. Staudt, Haibin Shi, Thomas E. Wales, Thomas E. Smithgall, John R. Engen, “Remodeling of HIV-1 Nef Structure by Src- Family Kinase Binding,” Journal of Molecular Biology, Available online 16 December 2017, ISSN 0022-2836, doi.org/10.1016/j.jmb.2017.12.008. 

 

SRC 3D Printing Technical Ceramics Webinar Registration

3D Printing Technical Ceramics Webinar

Topic SRC – 3D Printing Technical Ceramics Webinar
Description We are excited to invite you to our 3DCeram Seminar on using stereolithography and technical ceramic pastes to create mechanically strong and chemically resistant parts for a wide range of industrial applications.
Time May 27, 2020 12:00 PM in Eastern Time (US and Canada)

Speakers

custom photo of speaker
Peter Durcan
General Manager USA @3DCeram Sinto Inc.
Peter Durcan with over 3 years in additive ceramics, a bachelor of commerce degree holder with an MBA from the Open University, The United Kingdom. Peter, an Irish national who has been running the North American market for the past 18 months.

ABOUT SPECTRA RESEARCH

Spectra Research Corporation (SRC) offers a range of innovative high-quality scientific products and laboratory services to industrial and scientific markets throughout Canada. By selecting only the best products available and offering complete technical support, SRC is striving for continual improvement of our services and quality practices. If you require exceptional laboratory services and support, our technical expertise and industry knowledge allows us to provide service and training for all the products we represent.

You may choose in-house or web-based training and/or technical support for all our suppliers. Tailor-made programs are also available to meet your specific circumstances. We can also put you in touch with expert application scientists who will work with you directly.

Established in 1993, SRC is a subsidiary of Allan Crawford Associates (ACA), one of Canada’s largest distributors of electronic components, test equipment and integrated networking solutions.

SRC Apium 3D Printing Webinar Registration

Apium 3D Printing Webinar

Topic SRC – Apium 3D Printing
Description High performance polymers additively manufactured – basics and successful applications.

Apium 3D printers will be described and a short explanation of thermoplastics and their properties will be presented.

Time Jun 17, 2020 12:00 PM in Eastern Time (US and Canada)

Speakers

custom photo of speaker
Uwe POPP
Head of Sales & Research- Managing Dir. @Apium Additive Technologies GmbH
Uwe Popp is shareholder and Managing Director of Apium Additive Technologies GmbH . His previous experience includes Shareholder and head of research and development at Apium Additive Technologies GmbH, Head of research and development at Indmatec GmbH and Scientific assistant at KIT Institute of Applied Materials.
custom photo of speaker

Marc-André Fortin, Ing., Ph.D.
Professor of Biomedical Engineering at Université Laval

Marc-André Fortin has extensive experience in regenerative medicine and research.

  • Enseignement/Teaching: Pav. Adrien-Pouliot (bur.1745C); Université Laval, Québec G1V 0A6
  • Imagerie/Imaging: 2705, boul. Laurier (T1-61a), Québec, G1V 4G2
  • Biomatériaux/Biomaterials: E0-163, 10 rue de l’Espinay, Québec, G1L 3L5

ABOUT SPECTRA RESEARCH

Spectra Research Corporation (SRC) offers a range of innovative high-quality scientific products and laboratory services to industrial and scientific markets throughout Canada. By selecting only the best products available and offering complete technical support, SRC is striving for continual improvement of our services and quality practices. If you require exceptional laboratory services and support, our technical expertise and industry knowledge allows us to provide service and training for all the products we represent.

You may choose in-house or web-based training and/or technical support for all our suppliers. Tailor-made programs are also available to meet your specific circumstances. We can also put you in touch with expert application scientists who will work with you directly.

Established in 1993, SRC is a subsidiary of Allan Crawford Associates (ACA), one of Canada’s largest distributors of electronic components, test equipment and integrated networking solutions.

Last call to expand your knowledge in surface metrology guided

metrology guided
In the culmination of an incredible Month of Webinar Trainings, we invite you to join our Technologies Master Class next week!

During this final session, we will review and complement everything we have learned together these past few weeks and we will discuss the optical technologies developed and mastered by Sensofar with our most distinguished expert, our President & CTO Dr. Roger Artigas.

 Dr. Artigas PhD in physics (optical engineering) is deeply involved in the research and dissemination of 3D surface metrology methods and holder of several patents in the field, he is even member of the technical committee of the working group drawing up of the ISO 25178 standard: Geometrical Product Specification (GPS) – surface texture: Areal.  

All our expertise through a brief webinar training, specifically designed to help you learn more about the unlimited world of surface metrology.


OUR NEXT APPOINTMENT WILL INCLUDE  
Working principles and comparison of the main optical techniques for the surface metrology

surface metrology guided Guided by Dr. Roger Artigas

PhD in physics (optical engineering)

Holder of several patents in the field of optical surface metrology Member of the technical committee of the ISO 25178 Founder of Sensofar Tech SL in 2001

WEBINAR | 29 APR

Optical Metrology Techniques, the MasterClass!

We will explain everything we know for you.

We will simplify the complexity of the most used optical measurement technologies and provide you with a guide to know when and how to use them.

How much do you know about Confocal, Interferometry and Focus Variation?