Features at a glance
- Rapid and accurate particle size distributions
- Multimodal & unimodal size distribution software
- ISO 13321 and ISO 22412 compliant results
- Range > 0.3 nm to 6 µm
- Customizable reports
- Ideal for fast, routine sizing applications in research or quality control
- High power 35 mW diode laser
- Dynamic light scattering at 90°
- Temperature control, -5 °C to 110 °C
- Compact bench top unit, USB connection
- Zeta potential in low salt aqueous solutions/suspensions
- Accurate, rapid, easy to make zeta potential determination
- One piece, easy-fill disposable sample cells
- No cell alignment or calibration
- Zeta potential at 15°
- Temperature control, -5 °C to 110 °C
- Customizable reports, two parameter plots, graphical overlays
- Can resolve multi-modals
- Built in automatic procedures and parameters SOP
Brookhaven’s NanoBrook 90Plus Zeta instrument combines our NanoBrook 90Plus and NanoBrook ZetaPlus instruments into one versatile package for routine sizing and zeta potential analysis. It employs Dynamic Light Scattering (DLS) for particles/molecules size and Electrophoretic Light Scattering (ELS) for particles/molecules surface charge evaluation. This package not only allows rapid measurements of the effective size and zeta potential of samples, but also provides further information on multimode distribution in size and surface charge in polydisperse samples. Based on the proven technologies of Brookhaven, the NanoBrook 90Plus Zeta guarantees excellent mobility measurement performance in aqueous suspensions and low salt environments.
Simple fluids like water (low viscosity), glycerin (high viscosity) are Newtonian and exhibit viscosity effects, the dissipation of energy when particles move in such fluids. But dissolve macromolecules in these liquids –synthetic or biopolymers—and networks can form. In addition to viscosity effects, there are now elasticity effects, the storage of energy when embedded particle move. By following the mean square displacement (MSD) of tracer (probe) particles in such fluid and microrheological properties such as η*, the complex viscosity, G″, the viscous loss modulus, and G′, the elastic storage modulus, can be determined as a function of frequency.
Measurement of the autocorrelation function (ACF) using DLS techniques yields the MSD of tracer particles, which, under the right conditions, can be used to determine η*, G″, and G′ over a range of frequencies much higher than mechanical rheometers can attain. Much smaller sample volumes, in the microliters, are possible compared to mechanical instruments. Finally, since strains result from the thermally driven motion of tracer particles, these much smaller strains allow the study of fragile samples. The study of viscoelasticity in aggregating dilute protein solutions is a prime example of the benefits of DLS microrheology.
Reliable, Accurate, and Accurate Analysis
The new NanoBrook 90Plus Zeta particle analyzer incorporates all you need for fast, routine, sub-micron measurements of size and zeta potential. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and based on electrophoretic light scattering (ELS) for zeta potential measurements, most measurements only take a minute or two. Please see the 90Plus PALS for instruments combining sizing and PALS zeta potential.
Two Scattering Angles:
Size measurements will be made at the 90° scattering angle. Zeta potential measurements are always done using the 15° detection angle to minimize diffusion broadening.
Principles of Operation – Particle Sizing using DLS
Dilute suspensions, on the order of 2 ppm to 50 mg/mL are prepared, using suitable wetting and/or dispersing agents. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Two or three mL of suspension is required to make the measurements. Disposable, polystyrene square cells are used for aqueous and simple alcohol suspensions. Disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 90Plus Zeta.A square glass cell is available if desired and special small-volume cells may be used to reduce the volume of material needed to just 50 µL or 10 µL. For aqueous samples the 50 µL is a disposable cuvette. In all cases the sample is recoverable. For very small particles the high-sensitivity BI-APD detector is invaluable.