Features at a glance
- Rapid and accurate particle size distributions
- Multimodal & unimodal size distribution software
- ISO 13321 and ISO 22412 compliant results
- Range > 0.3 nm to 6 µm
- Customizable reports
- Ideal for fast, routine sizing applications in research or quality control
- High power 35 mW diode laser
- Dynamic light scattering at 90°
- Temperature control, -5 °C to 110 °C
- Compact bench top unit, USB connection
- Zeta potential for the difficult cases
- For proteins, peptides, mAb, RNA, and other biological samples
- For zeta potential in organic solvents
- For oily or viscous media
- For high-salt suspensions
- For samples near the I.E.P.
- 1,000 times more sensitive than other techniques
- Disposable cuvettes, no contamination or alignment
- Built in automatic procedures and parameters (SOP)
- Easy to use
Brookhaven’s NanoBrook 90Plus PALS instrument combines our NanoBrook 90Plus and NanoBrook ZetaPALS instruments in to one powerful package for sizing and zeta potential analysis. With over a decade of proven records in diverse applications, Brookhaven’s pioneer Phase Analysis Light Scattering (PALS) technology remains a step ahead of the competition. This package delivers unparalleled zeta potential (surface charge) measurement sensitivity and accuracy. It also provides unique real-time (in-situ) monitoring capability for electrophoretic response of particles/molecules under an electric field, a valuable tool ONLY from Brookhaven for delicate biological entities like proteins and exosomes. It has been successfully utilized in nearly every possible suspension environment from high salts such as PBS and sea water to organic solvents and of course aqueous solutions.
Simple fluids like water (low viscosity), glycerin (high viscosity) are Newtonian and exhibit viscosity effects, the dissipation of energy when particles move in such fluids. But dissolve macromolecules in these liquids –synthetic or biopolymers—and networks can form. In addition to viscosity effects, there are now elasticity effects, the storage of energy when embedded particle move. By following the mean square displacement (MSD) of tracer (probe) particles in such fluid and microrheological properties such as η*, the complex viscosity, G″, the viscous loss modulus, and G′, the elastic storage modulus, can be determined as a function of frequency.
Measurement of the autocorrelation function (ACF) using DLS techniques yields the MSD of tracer particles, which, under the right conditions, can be used to determine η*, G″, and G′ over a range of frequencies much higher than mechanical rheometers can attain. Much smaller sample volumes, in the microliters, are possible compared to mechanical instruments. Finally, since strains result from the thermally driven motion of tracer particles, these much smaller strains allow the study of fragile samples. The study of viscoelasticity in aggregating dilute protein solutions is a prime example of the benefits of DLS microrheology.
Reliable, Accurate, and Easy to Use
The new NanoBrook 90Plus PALS particle size and zeta potential analyzer incorporates all you need for fast, routine, sub-micron measurements of size and zeta potential. Based on the principles of Dynamic Light Scattering (DLS) for particle sizing and distribution, and based on phase analysis light scattering for zeta potential measurements; most measurements only take a minute or two.
Two Scattering Angles:
Particle sizing measurements will be made at the 90° scattering angle. Zeta potential measurements are always done using the 15° detection angle to minimize diffusion broadening.
Principles of Operation – Particle Sizing using DLS
Dilute suspensions, on the order of 0.0001 to 1.0% v/v are prepared, using suitable wetting and/or dispersing agents. A small ultrasonicator is sometimes useful in breaking up loosely-held agglomerates. Two or three mL of suspension are required to make the measurements. Disposable, polystyrene square cells are used for aqueous and simple alcohol suspensions. Disposable, glass round cells with reusable Teflon stoppers are used for aggressive solvent suspensions. Just a few minutes are required for the sample and cell to equilibrate with the actively controlled temperature environment inside the NanoBrook 90Plus PALS. A square glass cell is available if desired and special small-volume cells may be used to reduce the volume of material needed to just 50 µL or 10 µL. For aqueous samples the 50 µL is a disposable cuvette. In all cases the sample is recoverable. For very small particles the high-sensitivity BI-APD detector is invaluable.